Wagh 1, manoj mohan 2 and at shivashanmugam 2 1 ibss college of pharmacy, malkapur ms. Therefore, cfpc was used in this study to evaluate the in vitro and in vivo antidiabetic activity in stz induced diabetic rats. The intestinal digestive enzymes play a vital role in the carbohydrate digestion. Results and discussion in the preset study to investigate the antidiabetic activity of s1, s2 and s3 tested against alphaamylase enzyme. In vitro antidiabetic activity of leaves and seeds of. Aqueous extracts of the plants were prepared by maceration. Studies on invitro antiglycation potential of some. Assessment of in vitro antidiabetic activity of ficus. A dissertation submitted in partial fulfillment of the requirements for the degree.
Further, aqueous extract of terminalia paniculata bark contains active biomarkers which may possibly be responsible for the antidiabetic activity of terminalia paniculata bark. In vitro free radical scavenging and antidiabetic activity. The antidiabetic action of psidium guajava can also be attributed to the intestinal alphaamylase and alphaglucosidase inhibitory activity. Objectivethe study was carried out to evaluate invitro antioxidant and antidiabetic activity of methanol extract of medicinal plant piper cubeba l. Determination of in vitro antidiabetic effects of zingiber officinale roscoe 603 glucose diffusion inhibition the potential of ginger rhizome extracts to inhibit glucose diffusion into the external solution was investi gated at set time intervals gallagher et al. Their chemical structures, including absolute configurations were established on the basis of detailed physical data analysis methods viz. The ethanolic extract of amaranthus tristis linn leaves study exhibited potent hypoglycemic activity by in vitro studies. In vitroantidiabetic activity and glycemic index of bee honeys. Determination of in vitro antidiabetic effects of zingiber. Pdf antioxidant and in vitro antidiabetic activity of. Pdf in vitro antidiabetic activity of aqueous extract. Phytochemical and in vitro antidiabetic activity of. The results of inhibition of alpha amylase enzyme by the selected plant extracts have been represented in figure 1.
In vitro antidiabetic activity of caesalpina digyna r. The aqueous extracts from syzygium cumini seeds and psidium guajava leaves both showed a dose dependent inhibitory effect on alphaamylase activity karthic et al. The invitro antidiabetic activity of phoenix roebelenii leaf extract article pdf available in international journal of green pharmacy 111. In vitro antidiabetic and antioxidant activities of methanol extract of. In vitro antidiabetic activity of leaves and seeds of boerhavia diffusa in vitro alpha amylase inhibitory activity in vitro. In vitro antidiabetic activity a ecting glucose uptake in hepg2 cells following their exposure to extracts of lauridia tetragona l. In the in vitro antidiabetic studies were also performed so as to ensure the biological potency of the plant. Phytochemical analysis, anti inflammatory activity, in. Suksathan and borchs by glucose uptake method sangeetha d n and s. In brief, the 24 hr cell cultures with 7080% confluency in 40mm petri plates were allowed to differentiate by maintaining in. Zalb was found as significant active and inhibited in vitro ptp1b enzyme activity up to 57. The antidiabetic drugs reduce the gastrointestinal glucose production and absorption through the inhibition of carbohydrate digesting enzymes such as.
Their antioxidant activities were measured by 2,2diphenyl1picrylhydrazyl dpph free radical scavenging. The best concentration and time required to incubate glucose with hemoglobin was estimated. After the treatment of the yeast cells with the methanolic plant extracts, the glucose uptake was found to increase in a dose dependent manner. In vitro anti diabetic activity of hydroalcoholic leaf extract of s. In vitro analysis of the antidiabetic effect of aqueous extracts of the medicinal plants nigella sativa, eugenia jambolana, andrographis paniculata, gymnema sylvestre. In vitro and in vivo antidiabetic activity of isolated. Rajamani research scholar, bharathiar university, coimbatore 641 046, tamil nadu, india. Antidiabetic effect of plants and their active principles can be assessed in vitro using a variety of biological test systems. Tinospora sinensis, methanol extract, antioxidant activities. The antidiabetic and antioxidant activities of the ethyl acetatesoluble extract mfe of mulberry fruit morus alba l. The present study was therefore undertaken to investigate the antidiabetic activity and mechanism of action using various in vitro models designed to stimulate specific antidiabetic targets. In vitro cytotoxicity and glucose uptake activity of. In vitro and in vivo antidiabetic activity of lectin n. Both extracts were investigated for their inhibitory effect on.
From the study we revealed that the seed of the plant contains various classes of secondary metabolites and also the plants possess a moderate antidiabetic activity it terms of alpha amylase inhibition. The potential antidiabetic activity of the plant extract was evaluated against some carbohydrates. The in vitro antiglycation activity of extracts of some traditional antidiabetic plants was studied by determining the degree of non enzymatic hemoglobin glycosylation. In vitro glucose uptake assay glucose uptake activity of test drugs were determined in differentiated l6, 3t3 and vero cells. It is found growing in both natural and plantation forms in most of the parts of country. Invitro and invivo studies of antidiabetic activity of. The various kinds of phytochemicals were detected and then in vitro antidiabetic activity of p. Govindappa m 2015 n vitro and in vivo antidiabetic activity of lectin n acetylgalactosamine, 64 kda isolated from endophytic fungi, alternaria species from viscum album on alloxan induced diabetic rats ntegr besity iabetes, 2015 doi. Further, the antidiabetic activity of the extracts was compared with standard marketed antidiabetic drugs insulin and metformin table 4 and fig. There is no report was found on antidiabetic potential of this plant. Glucosidase evaluation of glucose absorption in vivo slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Mimosaceae,also known as khair, is a medium sized deciduous tree with crooked and forked trunk. Pdf in south africa, the number of people suffering from diabetes is believed to be rising steadily and the current antidiabetic therapies are. In vitro antidiabetic activity of leaf extracts of indianthus virgatus roxb.
In vivo and in vitro antidiabetic activity of terminalia. In vitro assessment of the antidiabetic activity of sclerocarya birrea and ziziphus mucronata. In vitro antidiabetic activity of cardiospermum halicacabum leaves extracts. In vitro antidiabetic activity and mechanism of action of. In vitro antidiabetic activity and mechanism of action of brachylaena. Pdf models to study in vitro antidiabetic activity of plants. Type 2 diabetes was induced in rats by streptozotocinnicotinamide 65 mgkg110 mgkg. The aims of this study were to determine the antidiabetic activity of aqueous extract of leaf le and fruit fe from p. In vitro antidiabetic activity of methanolic extract of citrus limon, punica granatum, musa acuminata peel pooja vasu, nazia d khan, zia h khan and sm mular abstract diabetes is a clinical syndrome characterized due to absolute or relative deficiency of insulin. They play a major role in evaluation of antidiabetic properties as an initial screening tool prior to in vivo studies. Evaluation of in vitro anti diabetic activity of selected. Pdf antidiabetic effect of plants and their active principles can be assessed in vitro using a variety of biological test systems. In vitro antidiabetic activity a ecting glucose uptake in.
Pdf models to study in vitro antidiabetic activity of. Dietary polyphenols, in addition to their antioxidant effects, have been reported to exert antihyperglycemic effects by binding to glucose transporters and competitive inhibition of digestive enzymes. The in vitro alphaamylase and alphaglucosidase inhibitory studies were performed using different concentration of extract and compared with a standard drug. Screening of in vitro antidiabetic activity of herbal. The present study was aimed to investigate in vivo, in vitro antidiabetic activity of aqueous extract of terminalia paniculata bark aetpb and characterize its possible phytoconstituents responsible for the actions. Antidiabetic and antioxidant effects and phytochemicals of. Invitro studies on insulin secretion, invitro studies on glucose uptake, studies using isolated pancreatic islet cell lines, assay of amylase inhibition and inhibition of. In vitro antidiabetic activity of ethanolic leaf extract of bruguiera cylindrica l. The in vitro antidiabetic activity investigation of the wogonin was carried out on digestive enzymes alpha glucosidase and alpha amylase, 3t3l1 preadipocytes and adipocytes. Methodsin vitro antioxidant activity of various concentrations of plant extract 20100. In vitro free radical scavenging and antidiabetic activity of aqueous and ethanolic leaf extracts.
Where v 0 is the initial rate of reaction, km is the michaelis menten constant, kmvmax is the slope, and the yintercept 1vmax was calculated using eq. In vitro antidiabetic activity of ethanolic leaf extract. In vitro antidiabetic activity and glycemic index of bee honeys 5 materials and methods foods, and possible implications of these differences sample collection a total of five multifloral honey samples in. Figures 1, 2 and 3 depict the % increase in glucose uptake by the yeast cell at different glucose concentrations i. In vitro antidiabetic activity by glucose uptake method in vitro, glucose uptake by l6 cells was measured to determine the effect of extracts on cellular glucose uptake behaviour. The plant material was extracted with methanol and the methanolic extract was screened in vitro for its antioxidant activity using abts and dpph assays. Standard acarbose showed a greater percentage inhibition of 80. The study reveals the presence of phytochemicals such as carbohydrate, tannin, flavonoids, phenols etc.
Pdf in vitro antidiabetic activity and mechanism of action of. In vitro antidiabetic activity of methanolic extract of. A higher antiinflammatory activity was observed in extract obtained from microwave extraction 64. In vivo and in vitro antidiabetic potentials of methanol. The present study was designed to investigate the glucose uptake of antidiabetic activity crude nhexane,ethanol, methanol and aqueous leaf extracts of cardiospermum halicacabum. This extract was used for in vitro and in vivo analysis of antidiabetic activity. Phytochemical evaluation and in vitro antidiabetic activity of. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. In vitro assessment of the antidiabetic activity of. In vitro antidiabetic activity of leaf extracts of. Pdf the invitro antidiabetic activity of phoenix roebelenii leaf extract. The aim of the current study was to screen the methanol extract of root of caesalpinia digyna for its in vitro antidiabetic activity. Frontiers evaluation of antioxidant, antidiabetic and.
Target guided isolation, in vitro antidiabetic, antioxidant activity and molecular docking studies of some flavonoids from albizzia lebbeck benth. The present study was aimed to investigate in vitro antidiabetic activity of aqueous extract of meshashringi formulation using enzyme inhibitory assays. In vitro study results scientifically supported the terminalia paniculata bark in vivo antidiabetic activity. Identification and quantification of bioactive compounds present was analysed by hplc. In vitro antidiabetic and antioxidant activities of.
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